Part:BBa_M1000:Design

Left mosaic end for Tn5 transpososome

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

BioBrick left end, TA cloning overhang

GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG

BioBrick right end, TA cloning overhang

GTT TCT TCC TGC AGC GGC CGC TAC TAG TA

mosaic end with cag upstream to insert PvuII cut site

cagCTGTCTCTTATACACATCT

mosaic end reverse-complement with ctg upstream to insert PvuII cut site

AGATGTGTATAAGAGACAGctg

mosaic left end in BioBrick form construction primer F

GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG cagCTGTCTCTTATACACATCT, MEL-F

mosaic left end in BioBrick form construction primer R

GTT TCT TCC TGC AGC GGC CGC TAC TAG TA AGATGTGTATAAGAGACAGctg, MEL-R

mosaic right end in BioBrick form construction primer F

GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG AGATGTGTATAAGAGACAGctg, MER-F

mosaic right end in BioBrick form construction primer R

GTT TCT TCC TGC AGC GGC CGC TAC TAG TA cagCTGTCTCTTATACACATCT, MER-R

Source

Synthesis from overlapping oligos

References

• http://epibio.com/intro_selection_guide_invivo_transposomics.asp

• http://epibio.com/applications_of_invitro_transposomics.asp